Dr. Eikelenboom w/JB - visual presentation re: touch dna....shows how finger touch top layers do not usually contain dna in nucleus....Epithelial cells often skin cells - difficult to detect these cells - alternate light source - wear orange goggles body fluids fluroesce...semen, blood, urine, but sweat from hands problem - sweat or warm outside...sweat from face or forehead - if does touch it once don't see it....also strive to see if we can see sweat from ALS - not detecting skin cells but trying to find sweat...sweat or could also be skin cells...lots times we don't find that ....working hypothesis...look @ where force applied...ie: rape case where shirt torn apart - where force applied on shirt - possible skin cells there must do the test to find....second problem...blood, saliva and semen in skin cells it is different - per lab 50% would be a high score if in lab........
how go about -
end up develop working hypothesis by looking @ scenario and type of contact....type of contact ....3 types of contacts - wear clothing ....rips in crime scenes and crimes...
wearing clothing rub against skin for a long time....could give profiles -highest chance of obtain profile....if suspect known to ...or in cases where find dna....forcible contact ie: strangle someone, grab them hard, apply force....top layer of skin cells down to cells that contain dna....slight touch ie: doorbell worse type of getting dna profile....some people shed more often than others....some people donate a good sample call a good shedder....sometimes we don't get enough dna cells to obtain a profile....very rough object with force go thru top layer of skin cells and grab material that contain dna....sticky material...ie: tape remove from clothing better than a smooth object.....
JB wants to tie into this case....a piece of duct tape....if a person cuts a piece of duct tape - sticky side and other side with other finger....if rip with bare hands....more than a slight touch....ever recover dna from duct tape? correct....sometimes get dna from non-sticky side? different types of sampling - duct tape if you know location where it was torn off possible - you can see tape is stretched...take sample obtain dna profile.....which side most prone to obtain profile....sticky side together with force applied....duct tape is known very strong adhesive....
sticky side of duct tape apply to someone face what would you expect to find? (object- overrule) robberies duct tape were ? for dna....what kinds of cells for face or mouth what kind of cell material gets on tape? lips, around mouth, epiththelial cells and buccal cells come from mouth area due to good to get dna material....
duct tape applied to this child's face - applied forcefully and sticky....opinion as to whether dna could have been collected (object- overrule)
yeah - tape put on mouth - was removed...about the face very likelyto get a dna profile....object- he respond tearing it off which is not a fact in this case....rephrase..
duct tape laced over person face and mouth - person placed in elements outdoors - expect to find dna? on the face would expect quite a lot of dna...circumstances in bad conditions dna can break down....with pcr techniques - could expect to find dna....
go to that portion of your presentation why you have that opinion...
