2011.06.16 TRIAL Day Twenty (Morning Session)

[JSR]

Wait I'm confused. She found the touch DNA on the duct tape or on some other piece of evidence. Sorry I'm doing more than one thing right now. I might have missed a part of the testimony.

 

[Softail]

JB is asking her about RFU thresholds. He is also asking about the peaks.

 

[okiedokietoo]

low copy? dna -
JB: read outs of dna
HS: swab put into tube, cells broken open to get to dna - taken thru process - multiple copies of, detect thru laser, put into dna profile
JB: what you get squiggly lines and peaks - 13 diff locations where you search for dna, markers, depending on how much dna

((((imo testimony with jb describing processes, to make dt appear scientifically knowledgeable

JB: threshold to exclude individual
HS: not shown on your chart - 50 rfu - peak where it begins to be detected
JB: below that artifacts

 

[Ssejors]

Way to go AZ!! you really shut up all the TH with your research! you rock!

Can someone please direct to me to what barrysgirl is refering to here?

AZ is my hero on WS. And I just got to work. What did I miss? barrysgirl?

 

[Mountain_Kat]

OMG, what does it all MEAN, Basil?

 

[Girlrilla]

I understand the jury are smart individuals....I have a master's degree and he lost me....they can't be following this that easily.

 

[1tasha]

JB: writing on poster board -
HJBP: ladies and gentlemen can you hear Mr. Baez?
JB: putting on mic - taking long time to put on mic - being shown how to use mic

Trying a capital case people!

 

[strawberry]

Is JB planning on selling his drawings on e-bay?

 

[Amster]

I was worried that HHJP would do this....bend over backwards for JB.

 

[ohiogirl]

I think it's so funny when Baez asks an expert witness to explain something to the Jury because he "doesn't want to get too technical", and "confuse the Jury".

As if Baez is on the same intellectual level with each expert, when it comes to each specific science. As if!

ITA!:crazy:

 

[Quiche]

(darn HLN, "you won't miss a thing!" then they go to three minutes worth of commercials, two minutes of other stories and recaps, then three more minutes in the courtroom!)

eta: then newsmakers! UGH

 

[nursebeeme]

he is trying to say that Casey didn't use the shovel (via no touch dna being there) but we already know she used the shovel LOL

 

[ahoyhoy]

My mind is wandering into oblivion.... : When JB gets to the real mike on the counsel stand does this mean we will hear a HUGE LOUD feedback SCREECH w/his mobile mike???

(waiting......)

I hope so.....ICA really deserves yet another laugh at her DP trial!

 

[wenwe4]

JB is miking up......lavellier mike (?)


low copy dna - perform dna testing know limited amount of dna - procedure performed to enhance more copies of the dna....add on molecular xeroxing....enhancing dna process to make more copies to generate a result...certain evals go into the results....don't have a lot of cellular material in the end ...must do things to have a reliable result.lo

if hold a firearm and pull trigger - touch dna - have skin cells -

low copy number is enhancing dna techniques....

readout on dna - end read out of profile ....back up more....dna process swabbing of the carpet the trunk .....swab put in tube...need to get to nuclear part of cell, add chemicals to extract dna out of item it is contained on.....PCR is xeroxing @ 13 locations of the dna and make multiple copies.....dna put thru capillary column .....thru a laser...signal dna is present with RFU's .....much like a EKG peaks on graph ....her end product is those peaks....now see end result to generate dna profile....get thru readout is electro....gram......(?) peaks most interested in.....13 diff locations search for dna...markers...points of interest....how much dna is there peaks indicate how much there RFU is higher collected and output into a peak....what are cut off levels for your lab for report dna type. ..threshhold 200 RFU's anything above 200 could use to make a match......other labs and other kits report lower levels? (object 0verrule) 200 level # determine by FBI validating instrument and kit @ the time in the unit....common in other labs sometimes threshold lower? other labs do have other threshhold limits.....any info falls below 200 RFU interpretation threshhold......there is one @ 50 RFu's peak label a peak......where a peak begins to be detected....other labs may use below 50.....threshhold for label a peak begins 50.....200 RFU threshhold conservative ......50 is based upon validation it is a scientific validation......give an indication something there @ 50 ....unless reaches 200 .....@ STR low side cannot be used below 200 Sex Typing....shovel and other items.....cannot report it.....doesn't mean some dna there but protocols prevent you (object-......earlier ruling Sidebar)

 

[Novice Seeker]

KBelichWFTV Kathi Belich, WFTV
Defense is trying to show the lack of enough dna on the shovel to profile means Casey couldn't have used much force with it or not used it

or that ICA used gloves...

 

[BigFatMommyDog]

does anyone else find it odd that there was ABSOLUTELY no dna found - considering how many people had access to the car (as JB drilled into us previously)? None, not ICA, CA, GA, LA, SP ... a 10 year old car blah blah blah.

No DNA at all. Hmm. ;-)

 

[KenoshaKid]

When you say you can't report it, it doesn;t mean there's nothing there, correct?

JA's objection overruled, now sidebar.

 

[whiteangora]

I feel so sorry for the jury, I'm sure they are lost in this mystical land of Baez and his useless information. After this witness steps down they will forget every word she said....Except for when JA asks her Real Questions and there is a solid answer or two. moo

 

[countzero]

I wish the nun I had in grade school, Sister Mary Catherine, was there to smack JBs hands palm side out ... she did not put up with any nonsense. I heard it really, really hurts.

 

[passionflower]

blood DNA............a waste, a HUGE waste!
JB told us baby Caylee 'drowned'....there would be no blood!