Please provide a link for luminol degrading TMB...
Not doubting you just would like to read it. tia
The rest of your post I agree with..
This is where I found a clear, concise explanation about the different uses, sensitivities and limitations of Luminol and TMB. I have collected information from various parts of the report and summarized it below.
Luminol Accuracy
Sensitivity
− 10‐6 to 10‐8: most sensitive presumptive test (10 to the power of -6, or -8)
Specificity
− Many false positives: bleach, metals, chemical oxidants, vegetable peroxidases
− Will not detect differences in animal or human blood
http://projects.nfstc.org/bsw/presen...10_CBS_JMS.pdf page 41
Luminol –Limitations
Stability
− Very unstable
− About eight hour limit
Mostly used at crime scene
− Can dilute out stain (possibly too much for DNA analysis)
− Used more for blood spatter, crime scene reconstruction
same link; page 42
TMB Sensitivity
Sensitivity
− 1:10,000 on dried stains
Specificity
− Not as specific as KM test
− False Positive to vegetable peroxidases, bleach, otassium permanganate
− Will not detect differences in animal of human blood
Kastle‐Meyer Test –Limitations
Kastle‐Meyer (Phenolphthalein)
TMB is not as specific as KM test
Sensitivity
− 1 in 1000 on dried stains
Specificity
− Can weed out false positives between steps 2 and 3
− Chemical oxidants, vegetable peroxidases
− Will not detect differences in animal or human blood
Stability
− Relatively stable if the reagents are stored separately and refrigerated
http://projects.nfstc.org/bsw/presentations/02_BioScreening_Blood_012010_CBS_JMS.pdf
http://projects.nfstc.org/bsw/
10-6 would be 10 to the power of negative 6, which is 1:1,000,000 or 0.000010
10-8 would be 10 to the power of negative 8, which is 1:100,000,000 or 0.00000010