I think Otto that standard operating procedures/protocols are essential whether it is for DNA testing, driving an automobile, or whether we are posting right in this forum.
The purpose of these procedures/protocols ensure that there are checks and balances in place to avoid consequences further down the road.
If you are driving an automobile you must know the signs, drive in the proper lane for everyones safety, not drive while impaired, and have a valid drivers license just to name a few. The consequences of abusing any of these can be and are deadly in various scenerios.
The same holds true in DNA testing. These protocols/procedures are put in place so that issues such as we are discussing here do not arise.
LCN DNA is still controversial first I believe because people need to become more educated with respect to it. It is also much more costly to operate as these labs must have various sterialization procedures in place. I believe there is a place for LCN DNA in forensic science but what I do not want to see is people performing these tests without the knowledge, without the proper environment, and every individual must recognize that there are still limitations even with respect to LCN DNA. If we are testing a sample of 200 picograms I would personally assume that there is a greater chance for a more accurate profile. If you are talking 5 picograms, I would have to seriously review the testing methodology as I have not seen where you can get a valid profile with these numbers. Maybe with the advancements we are seeing in technology this will become a mute point but till the world as a whole accepts these procedures/protocols we are going to continue to run into issues.
One of the biggest misconceptions is that people think that in LCN DNA all the DNA is used. Although technically correct, part of the protocol is that it must be amplified so that a remaining sample that has been amplified can be reviewed and saved.
Stephanoni as I have pointed out did not use any sort of scientific protocol/procedure normally used. It is not even in the scientific writings on this. The nine areas that Stephanoni did not conform to standard protocols/procedures were:
1. The DNA wasnt amplified enough; the very weak fluorescence was simply blown up.
2. The test site was not remote from other DNA tests to avoid contamination.
3. Specialized LCN-quality entry procedures to avoid contamination were not used.
4. A positive pressure environment was not maintained to exclude contamination.
5. Special LCN sterilization procedures to destroy errant DNA were not used.
6. The entire sample was consumed in a single test; no comparison of tests was possible.
7. No sample was retained for future reference. The test can never be reproduced.
8. No negative control tests were run to check for contamination.
9. No control tests to check for field contamination were performed
http://www.sciencespheres.com/
http://freeaman.001webs.com/pdfs/LCN_DNA_II.pdf
I did read the article you cite with great interest. What we do not know in these particular cases is how low the DNA was. Was it 200 picograms or 5 picograms? It appears that rulings were made based on the validity of the both the samples and testing protocols and procedures.