I'm nervous. This is my first post here so please be gentle.
I've found a site where JH posted about the results of one of his research projects. I've asked a friend to basically tell me what he means and, basically, what he says is that the review means that the specific research project failed. And, he added, that a project like this would have been a major project and would have been a major blow to JH since it meant months of tedious work and research for naught, basically. I have no idea. I don't understand most of it.
What I did notice, however, is that if this is truly JH as he indicates on the site, his review of the research was posted in May 10, 2012 and was posted by "James Holmes" of "UC Denver." And, as such, could be our first indicator in the downward spiral he experienced academically and mentally prior to the June withdrawal.
I've quoted his review here...
Effective transferability to mESCs questionable
Application or Technique: Neural Stem Cell Differentiation
Cell Start attempted to be used in place of MEF feeder layer to maintain mESCs. Attached and grew on substrate. After applying multiple neural stem cell differentiation protocols, cells were not Sox1 GFP+ (neuronal stem cell marker). Suggests MEF provides some yet unknown factors in mESC maintenance not seen in Cell Start.
Link:
http://reviews.invitrogen.com/1160/A1014201/reviews.htm