JA w/Dr Vass
2 high peaks.....highest was chloroform....chloroform is a decomp event compound in human decomp......reason we progressed was the result of chloroform shockingly high.... (object-overrule).....small peaks noted....tried to concentrate air sample.....used cryotrapping to concentrate....present w/Dr. Wise while analysis being done....removed piece of carpet from metal can....not sure if air in can was representative (object-ovrrule) what coming off the carpet....placed carpet in plastic tevlar bag- polyvinyl bag....placed carpet in bag and incubated it @ body temp....tevlar bag won't change sample.....incubate it because carpet sample came from trunk of car in FL anticipated quite warm - high 90's close to 100 degrees in trunk in FL, speeds up evolution of these compounds...estimated 2 days in analysis....
take air sample of bag - from that bag extrated 10 ml, thru cryo....to trap.....
identify sample not concentrated - present for all the post analysis......JB- almost everyone...(object-overrule)
51 indiv. chemical components out of 10 mil extracted from tevlar bag - chloroform present.....GCMS - chromatogram.....large peaks and tall peaks ...much more concentrated amount of material vs. small peak.....
largest peak called base peak ....base peakwas chloroform.....surprised? Shocked! never seen chloroform in those levels in 20 years......in human decomp have seen chloroform in parts per trillion ...very very small amounts.
attempt to quantify amount of chloroform - shot a standard - an analite.....chloroform ....to ensure it was chloroform....concentration of standard is known......rough approx. of (object- speculation - ovrrule)
chloroform was in the parts per Million range vs. Trillion range..
attempt to quantify.....need to purchase much more concentrated standards.....not more helpful to further test @ minimum........chloroform high rate of evap. much like gas.....(object-ovrrule) appears in top trunk first, @ tow yard, lower range in A' garage....lower in OCSO garage.......minimum range....
obtain a standard of the carpet from similar vehicle......
GCMS from control carpet and A's carpet.....enter demonstrative chart.....object-not testified to how obtained...who conducted test and reliabiitly...HHBP who conducted test ...both Dr. Wise and Dr. Vass conducted = HHBP data you reasonably rely upon for data in this case? Yes......Admitted into evidence
output of actual GCMS - which peak represents chloroform = tallest peak - @ 16 min.....(appears to go off the chart?) numbers @ left = abundance - rough approx. of how much material is present......top number 16 million in terms rough estimate how tall that peak......above 16millionish range.....sample from carpet from vehicle in this case.....also show other peaks....region @ lower right side compounds associated with gasoline....dozens of components ......benzy