The right fingernails indicated that two further unique profiles were present, unidentified male #2, and a unique unknown female profile. (JonBenet could not be excluded as a contributor)
The waistband, seams, and crotch of panties (Distal Stain 007-2) CODIS all matched and produced the profile that has been entered into the CODIS database, unidentified male #3 (Strength/weakness of profile: 10 markers)
The above profiles were determined through typical STR DNA testing.
Touch DNA (TDNA) testing, all presumably done at the Bode facility revealed one matching profile and a further two unique profiles, both male:
TDNA on the waistband of leggings matching DS 007-2 male #3
TDNA on the wrist bindings – male #4 (Strength/weakness of profile: 6 markers)
TDNA on the “garrote” – male #5 (Strength/weakness of profile: 7 markers)
(Also, TDNA on the pink Barbie nightgown found in the Wine Cellar with the body of JonBenét was identified as belonging to BR and PR.)
A full CODIS profile has 13 markers; any profile with fewer markers is a partial profile. All DNA profiles in this case are partial profiles
The highest quality DNA, and the only profile in this case that has been entered in the CODIS database, at 10 markers, is Distal Stain 007-2
All other DNA is weaker, in other words, less markers.
Kolar’s book confirmed the speculation that the profile from one of the blood spots that eventually ended up in CODIS originally had only 9 markers.
The male DNA sample, subsequently identified as Distal Stain 007-2, only contained 9 genetic markers, and like the DNA collected from beneath JonBenét’s fingernails, was of insufficient strength to be entered into the state and national databases. Moreover, the sample was so small that technicians were not able to identify the biological origin of the exemplar.
Foreign Faction, Who Really Kidnapped JonBenet, James Kolar, page 140
Eventually a 10th marker was identified which then met the minimum standard for entry into CODIS:
DNA replication technology was utilized in the Denver Police Department’s crime lab, and the 10th marker was eventually strengthened to the point that the unidentified male sample discovered in JonBenét’s underwear was able to be entered into the state and national databases. This laboratory success didn’t take place until 2002, nearly 6 years after the murder of JonBenét
Foreign Faction, Who Really Kidnapped JonBenet, James Kolar, page 140
I met with the man who had worked so diligently to enhance the DNA sample identified as Distal Stain 007-2. Denver Police Department crime lab supervisor Greg Laberge met me for lunch in early December 2005 and advised me that the forensic DNA sample collected from the underwear was microscopic, totally invisible to the naked eye. So small was it in quantity, consisting of only approximately 1/2 nanogram of genetic material, equivalent to about 100 – 150 cells, that it took him quite a bit of work to identify the 10th marker that eventually permitted its entry into the CODIS database.
Foreign Faction, Who Really Kidnapped JonBenet, James Kolar, pages 303 - 304
The profiles found from the fingernail clippings of JonBenet were presumably not from the non-sterile nail clippers that the coroner was in the habit of using.
DNA revisited in light of James Kolar’s book - Forums For Justice
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All profiles in this case are partial
