Intruder theories only. No posts from rdi members allowed

[ScarlettScarpetta]

Here is my issue, Augmented means to change something..

When I google this it comes up as nothing called Augmented dna.

So if you could explain what you are saying with a reference that would be helpful. From what I see they got 6 profiles that are unknown.

 

[Deleted member 39678]

I've never heard of "augmented DNA" myself.

 

[ScarlettScarpetta]

Well apparently there is something scifi about it, But I will be darned all the journals, Websites and nothing about augmented DNA. Mixed profiles, partial profiles but not ADNA.

 

[Deleted member 39678]

Well apparently there is something scifi about it, But I will be darned all the journals, Websites and nothing about augmented DNA. Mixed profiles, partial profiles but not ADNA.

Sci-fi? Well, that wont help us.

 

[ScarlettScarpetta]

I found the term in dvd's books and scifi sites..

I looked far and wide also. I hope there will be clarification.

 

[BOESP]

Incomplete DNA samples have to be replicated or augmented. Incomplete samples do not contain enough markers to be of use to CODIS unless they are replicated or augmented.

 

[ScarlettScarpetta]

Incomplete DNA samples have to be replicated or augmented. Incomplete samples do not contain enough markers to be of use to CODIS unless they are replicated or augmented.

Okay.. Do you know the process they use to do this? Or how much they need to replicate? Thanks for the explanation..

 

[Dewey2Me1MoThyme]

Incomplete DNA samples have to be replicated or augmented. Incomplete samples do not contain enough markers to be of use to CODIS unless they are replicated or augmented.

I thought they did that when there was not enough of the sample to test? Maybe I'm confused on what you are trying to say. If they lack enough markers, how can more markers be added to get an accurate DNA? I'm not trying to be argumentative, just trying to get my head around this.

 

[Deleted member 39678]

I think that your on to something Dewey. Maybe BOESP is talking about DNA amplification or PCR. It's how they get a large enough amount of DNA to do comparisons and other analyses. It's not adding anything, it's duplicating DNA.

What is PCR?

Sometimes called "molecular photocopying," the polymerase chain reaction (PCR) is a fast and inexpensive technique used to "amplify" - copy - small segments of DNA. Because significant amounts of a sample of DNA are necessary for molecular and genetic analyses, studies of isolated pieces of DNA are nearly impossible without PCR amplification.

http://www.genome.gov/10000207

 

[BOESP]

Mea culpa. Yes, amplification is also correct.

Carry on.

 

[BOESP]

I thought they did that when there was not enough of the sample to test? Maybe I'm confused on what you are trying to say. If they lack enough markers, how can more markers be added to get an accurate DNA? I'm not trying to be argumentative, just trying to get my head around this.

Dewey, the DNA was not of the quality to be submitted to CODIS so it had to amplified/replicated/augmented (pick a word...any word) before CODIS would accept it. Iirc, JonBenet's sample contained only nine markers and CODIS needs thirteen.

Now, pardon me boys but I hear the Chattanooga ChooChoo and I need to catch it.

 

[Deleted member 39678]

Dewey, the DNA was not of the quality to be submitted to CODIS so it had to amplified/replicated/augmented (pick a word...any word) before CODIS would accept it. Iirc, JonBenet's sample contained only nine markers and CODIS needs thirteen.

Now, pardon me boys but I hear the Chattanooga ChooChoo and I need to catch it.

Where is this information about quality coming from? Are you saying that PCR will add markers to a DNA sample?

 

[2 percent]

Where is this information about quality coming from? Are you saying that PCR will add markers to a DNA sample?

It was widely reported that the DNA sample from the underwear only had 9 markers and a weak tenth. FBI CODIS requires 13 markers for a full profile. To reach the required level the DNA obviously had to be punched up.

I've never heard how this was achieved. I only know the DNA mysteriously went from 9 to enough-to-be-run. Maybe someone with more information will be able to help both of us

 

[renah]

Attention RDIs!! Per Kimster, this thread will be for IDI theories and discussion ONLY! We now have our own RDI ONLY thread! arty:

Let's respect this thread and not discuss/argue our RDI theories here, and ask that the IDI not discuss/argue their theories on our thread!

Pass the word! See you there!

Well what if someone hasnt made up their mind yet, lets say me? It almost seems like i cant post or discuss on either thread... Do we need a third for an Undecided Poster category?

 

[Roy23]

This is an IDI thread. All anyone needs to know about the DNA is that is accepted by the strict Federal Codis guidelines as complete enough to be in the database. It wasn't forced there by IDI's --It is real in the eyes of the law.

 

[2 percent]

Well what if someone hasnt made up their mind yet, lets say me? It almost seems like i cant post or discuss on either thread... Do we need a third for an Undecided Poster category?

The separation of the subjects is to keep harmony right now.
I can see where you may think it is exclusionary to people without a firm theory in this case but that's just not so.

Think about it...what is this site about first and foremost?
Debate and learn or teach.
To debate there has to be discussion.
To learn you have to see facts and theories
To teach you have to speak up.

I think you should discuss your RDI beliefs in that thread and your IDI theories in that thread and anything in between, start your own thread.
:grouphug:

 

[Deleted member 39678]

I found this on the FBI's web site about CODIS and the National DNA Index System or NDIS.

Q: What are the minimum loci requirements for the STR DNA data submitted to NDIS?
A: The minimum CODIS Core Loci required for submission of DNA data to NDIS vary by specimen category. Generally, the 13 CODIS Core Loci are required for submission of convicted offender, arrestee, detainee, and legal profiles. The 13 CODIS Core Loci and Amelogenin are required for relatives of missing person profiles.

All 13 CODIS Core Loci must be attempted for other specimen categories with the following limited exceptions:

For forensic DNA profiles, all 13 CODIS Core Loci must be attempted but at least 10 CODIS Core Loci must have generated results for submission to and searching at NDIS.
For Missing Person and Unidentified Human Remains, all 13 CODIS Core Loci must be attempted.

The minimum number of markers needed for forensic DNA profiles to be submitted to NDIS is 10.

So now I need to find out exactly how many markers the DNA sample in question actually has in it.

http://www.fbi.gov/about-us/lab/biometric-analysis/codis/codis-and-ndis-fact-sheet

 

[2 percent]

This is an IDI thread. All anyone needs to know about the DNA is that is accepted by the strict Federal Codis guidelines as complete enough to be in the database. It wasn't forced there by IDI's --It is real in the eyes of the law.

BBM.
I'm curious, why is that the only thing anyone needs to know?

 

[Roy23]

I found this on the FBI's web site about CODIS and the National DNA Index System or NDIS.


The minimum number of markers needed for forensic DNA profiles to be submitted to NDIS is 10.

So now I need to find out exactly how many markers the DNA sample in question actually has in it.

http://www.fbi.gov/about-us/lab/biometric-analysis/codis/codis-and-ndis-fact-sheet

Well it is obviously at least 10. Why are we debating a DNA that is accepted by the Codis guildelines. Its real.

 

[Deleted member 39678]

Well it is obviously at least 10. Why are we debating a DNA that is accepted by the Codis guildelines. Its real.

You do have a good point. I would assume that it has least 10 markers. I was just going to take a look for myself since I was given faulty information about the minimum number of markers needed as being 13. Thanks.