The police and the dna lab say the dna belongs to someone. I’ve included the top part of the report so you can identify the report and the date it was done and I’ve included the page where they refer to him as an unknown male. He has an identity of “unknown male”.
Last of the many DNA debates
Clouded Truth answered the same redundant DNA post just a few weeks ago. From now on, I'll just cut and paste.
The serological testing on the panties for amylase was inconclusive. Amylase is also found in urine, and the panties were soaked in urine which may very well have impacted the results of the test.
A full profile would consist of 14 alleles. One allele was found in JB's panties. Hardly conclusive as to being from foreign source.
I think it's also important to note that even if the CODIS database ever comes up with a match, which it has not in all these years, that identified person would still need to be fully investigated before any charges could even be considered. The DNA evidence in this case is not even close to proving there was an intruder. Does it create doubt? Yes. But not enough to hang your hat on IMO.
claimed to learn from talking to LaBerge who did the final 13STR testing that identified enought markers to put the profile into CODIS. It does seem like Kolar misunderstood whatever LaBerge told him, because there had been multiple earlier tests of the DNA that had consumed plenty of it.
DQA1/polymarker test by CBI in Jan 1997 - minimum requirement 2 nanograms.
D1S80 test by CBI in jan 1997 and Cellmark in Feb 1997 - consumes between 0.5 and 40 nanograms (can be very wasteful)
13STR by CBI in Sep 1999 - the kits used consumed 1 to 2.5 nanograms
13STR by Denver Police Forensics Lab (LaBerge) in 2001 - again 1 to 2.5 nanograms
Basically, if LaBerge told Kolar there was 0.5 nanograms of unidentified male DNA, he had to have meant there was 0.5 nanograms remaining.
CloudedTruth said:
A 2017 study was done, an investigation of DNA transfer onto clothing during regular daily activities by Ruan, et al. Researchers took freshly laundered shirts from 50 participants and tested the DNA in several areas, then gave the shirts back to be worn for a day while doing their regular routine. And while the amounts of DNA increased significantly after wearing, they were surprised to find many interpretable foreign DNA samples BEFORE the shirts were even worn. In some cases, the owner of the clothing was not even the predominant DNA profile, a clear indication that background levels of DNA even on clean clothing can contain significant amounts of foreign DNA. Most of the samples taken after having worn the shirts had two to three mixtures of different people being the most common.
They did further testing on cotton swatches that were laundered with participants clothing in a typical laundry cycle.
In this study the average amount of DNA that accumulated on a previously pristine cotton swatch through one laundry cycle was 1 nanogram.
The authors of the study concluded that, "the results of this study reaffirm that any DNA profiles taken from from casework garments should be treated with extreme caution with regards to their case relevance".
A shout out to Redditor straydog77 for finding this study.
Click to expand...
Of course, there had been significantly more than 1 nanogram of UM1 DNA as per above. No garment ever yielded more than a tenth of the foreign male DNA on JonBenet (Kolar, Foreign Faction).
CloudedTruth said:
The serological testing on the panties for amylase was inconclusive. Amylase is also found in urine, and the panties were soaked in urine which may very well have impacted the results of the test.
In the CBI report from Jan 10 1997, object 14I (foreign stain swabs from the sexual assault kit) indicated the presence of amylase, which is found in saliva at concentrations a thousand times higher than urine.
CloudedTruth said:
A full profile would consist of 14 alleles. One allele was found in JB's panties. Hardly conclusive as to being from foreign source.
One? Many, many more were found.
the-facts-about-dna-in-the-jonbenet-case-v0-0zcoum27jw8c1.png
CloudedTruth said:
I think it's also important to note that even if the CODIS database ever comes up with a match, which it has not in all these years, that identified person would still need to be fully investigated before any charges could even be considered. The DNA evidence in this case is not even close to proving there was an intruder. Does it create doubt? Yes. But not enough to hang your hat on IMO.